I. Platform for Species Features Analysis and Identification
(1) solve the problems when hybridization is beyond reach because of the differences in chromosomes sizes and the number of genomes between Phalaenopsis species;
(2) evaluate confusing or similar species;
(3) construct fragrance spectrum of orchids and the databank;
(4) inspect sub-seedling mutation rate;
(5) build identification barcodes;
(6) build species certifying platform.
(I) Parents Pregnancy Analysis and Evaluation on Polyploids Induction of Orchids
Orchid farmers who encounter bottlenecks in hybridization breeding
When Phalaenopsis hybridization is beyond reach because of the differences in the chromosomes sizes and the number of genomes between Phalaenopsis species, great limits are imposed on the breeding of innovative species. Our team, therefore, provide the breeding technology of polyploids, when accompanied by the polyploids analytical modules established by the genetic research center, we help the farmers understand the number of genomes and estimate the pregnancy rate in order to increase the opportunity of cultivation.
In addition, for species that could not be combined because of the number differences in genome, this alliance also provides the polyploids induction technology and help farmers to polyploidize the genome of excellent parents, solving problems like triploids
(1) Orchid Parent Pregnancy Rate Analysis – Examining DNA amount in orchid cores and the number of ploids.
(2) Polyploids induction – inducing diploids or triploids into tetraploids and hexaploids that are prone to pregnant.
(II) DNA molecule marking technology in orchid species identification
(2) Orchid farmers who are interested in establishing molecular quality management system
(3) Orchid farmers who would like to establish DNA Molecular Identification Barcode with DNA Fingerprint Identification Map – to be used in the protection of species rights and pattern application.
DNA Molecular Marking & Identification Technology has in recent years been widely applied in identifying the species of crops. The Orchid Molecule Identification Platform uses 10 sets of florescence SSR molecular markers, accompanying with an automatic capillary DNA sequencer to build an effective, precise and highly stable SSR identifying system for Phalaenopsis molecular markers, which can be used to identify huge number of Phalaenopsis species and genomes. This can be used to create unique DNA molecular identification barcode and sort out the issues on confusing species while at the same time, it could be used in patterned species application or relevant evidence in patterned species violation cases.
(1) Species Identification: Molecular quality control identification for similar species and
(2) DNA fingerprint map detection and identification: Building the DNA fingerprint map for variety specificity.
(III) Orchid Fragrance Map Detection Technology
Businesses that want to know more about the fragrant ingredients of orchid flowers
Many businesses own large quantity of fragrant Phalaenopsis orchid breeding maps, but they have no idea what parent contribute what fragrant ingredient.
Our research team has established a set of extracting and analyzing technologies for fragrant flowers, which steadily and speedily extract fragrant ingredients from various orchid dpecies to be analyzed and systematically build a databank of fragrant Phalaenopsis. It digitalizes and clarifies data of hereditary fragrant properties and will be able to help businesses breed precisely specific Phalaenopsis with specific fragrances.
(1) Extraction and Analysis of Fragrant Properties: Extracting fragrant ingredients and building a map of them.
(2) Hereditary fragrant properties analysis: comparison of the fragrance maps of the parents and those of the progenies.
(IV) Mutational Rate Detection in Mericlones
Businesses that would like to detect early if the Phalaenopsis generate mutations in the mericlones
The asexual reproduction with bud balls can easily develop mutations, yet the high cost in reproducing through orchid buds lowers the competitiveness in the market. Our research team has developed a set of 17 detecting chips for genomes (the genomes can be early molecule markers for detecting mutations). It can effectively and precisely detect mutations in Phalaenopsis and help farmers select the varieties they need for further cultivation. Furthermore, this technology can be used to help businesses establish the best tissue-cultivating system and ensure the quality homogenization of orchid seedlings.
Gene detection through chips